FD NeuroApop™ Kit 神經(jīng)元凋亡檢測(cè)試劑盒
簡(jiǎn)要描述:
FD NeuroApop™ Kit 神經(jīng)元凋亡檢測(cè)試劑盒(Tunel法):FD神經(jīng)元凋亡檢測(cè)試劑盒(FD NeuroApop™ Kit)提供的試劑和步驟都經(jīng)過(guò)優(yōu)化以能在特異性和靈敏度上高效檢測(cè)凋亡神經(jīng)元,且維持極低背景。此試劑盒適用于冰凍和石蠟切片,以及培養(yǎng)細(xì)胞。本試劑盒整個(gè)操作流程約需4h。
產(chǎn)品時(shí)間:2023-02-15
FD NeuroApop™ Kit 神經(jīng)元凋亡檢測(cè)試劑盒(Tunel法)
搜索關(guān)鍵詞:
FD NeuroTechnologies, Inc.;Golgi-Cox impregnation 浸染法;Neuronal apoptosis 神經(jīng)元凋亡;Fluoro-Jade B;In situ DNA nick-end labeling (TUNEL) 原位缺口末端標(biāo)記法;
產(chǎn)品描述:
FD NeuroTechnologies, Inc(USA)公司生產(chǎn)的FD神經(jīng)元凋亡檢測(cè)試劑盒(FD NeuroApop™ Kit)特別研發(fā)用于中樞神經(jīng)系統(tǒng)組織切片中的神經(jīng)元凋亡檢測(cè),基于原位DNA缺口末端標(biāo)記技術(shù)(TUNEL法)。該技術(shù)使用末端脫氧核糖核酸轉(zhuǎn)移酶(TdT)催化*偶聯(lián)的脫氧*插入DNA pian段自由的3’-OH末端,其被認(rèn)為是凋亡zui典型特征之一。之后插入DNA的*使用親和素-*復(fù)合物(ABC)方法得以信號(hào)擴(kuò)大,并在光學(xué)顯微鏡下進(jìn)行可視化鑒定。
FD神經(jīng)元凋亡檢測(cè)試劑盒(FD NeuroApop™ Kit)提供的試劑和步驟都經(jīng)過(guò)優(yōu)化以能在特異性和靈敏度上高效檢測(cè)凋亡神經(jīng)元,且維持極低背景。此試劑盒適用于冰凍和石蠟切片,以及培養(yǎng)細(xì)胞。本試劑盒整個(gè)操作流程約需4h。
試劑盒組分(PK201):
PK201(For 35 sections) | Storage | ||
1 | Digestive Enzyme 消化酶 | 2 ml x 4 | -20℃ |
2 | Reaction Solution A 溶液A | 2 ml x 2 | -20℃ |
3 | Reaction Solution B 溶液B | 85 µl | -20℃ |
4 | Reaction Solution C 溶液C | 60 µl | -20℃ |
5 | Chromogen Solution 顯色液 | 20 ml | -20℃ |
6 | Equilibration Buffer 平衡緩沖液 | 20 ml | +4℃ |
7 | Detection Reagent 檢測(cè)試劑 | 5 ml | +4℃ |
8 | 10x Phosphate-Buffered Saline 磷酸鹽 | 250 ml x 2 | +4℃ |
應(yīng)用實(shí)例:
圖2. Detection of apoptotic neurons with FD NeuroApop™ Kit. Paraffin section (10µm) cut from the inferior colliculus of a mouse embryo (E17). The section was processed with FD NeuroApop™ kit and counterstained with methyl green. Sections courtesy of Drs. Michael Vogel and Lisa Qiu.
貨號(hào) | 名稱(chēng) | 規(guī)格 | 價(jià)格(元) |
PK201 | FD NeuroApop™ Kit FD神經(jīng)元凋亡檢測(cè)試劑盒 | For 35 sections | 7176 |
PK101 | FD Apop™ Kit Tunel凋亡檢測(cè)試劑盒 | For 50 sections | 6118 |
技術(shù)文獻(xiàn):
[1] Gavrieli Y, Sherman Y, and Ben-Sasson SA. (1992) Identification of programmed cell death in situ via specific labeling of nuclear DNA fragmentation. J. Cell Biol. 119:493-501.
[2] Wyllie AH. (1980) Glucocorticoid-induced thymocyte apoptosis is associated with endogenous endonuclease activation. Nature. 284:555-6.
[3] Arends MJ, Morris RG, and Wyllie AH. (1990) Apoptosis: the role of the endonuclease. Amer. J. Pathol 136:593-608.
[4] Hsu SM, Raine L, and Fanger H. (1981) Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures. J. Histochem. Cytochem. 29:577-80.
— —Written/Edited by V. Shallan【版權(quán)歸MKBio懋康所有】
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FD NeuroApop™ Kit 神經(jīng)元凋亡檢測(cè)試劑盒(Tunel法)
FD NeuroApop™ Kit 神經(jīng)元凋亡檢測(cè)試劑盒(Tunel法)
FD NeuroApop™ Kit 神經(jīng)元凋亡檢測(cè)試劑盒(Tunel法)